Neuroscience in Denmark


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General information

Name (center, department, group or other)
NeuroMet
Contact name
Helle Waagepetersen
Contact email
helle#dot#waagepetersen#at#sund#dot#ku#dot#dk
Contact title
Professor
Date
10. May 2016


Brief description of research activities


How are brain cells regulating their energy metabolism?
How are neuroransmitter pools of glutamate and GABA maintained?
How are neurotransmitter homeostasis and brain energy metabolism affected in Alzheimers disease and Type 2 diabetes ?
... and how may we manipulate these pharmacologically?

These are the four questions driving research in NeuroMet

The research in NeuroMet is focused on energy and amino acid metabolism in the mammalian brain. Primary mouse cell culture systems of neurons and astrocytes from cerebral cortex or cerebellum are extensively employed. In addition, genetically modified animals, organotypical hippocampal cultures, acutely isolated brain tissue, isolated mitochondria and cell lines are applied as model systems. An array of 3H, 15N and 13C isotopes is utilized in the mapping of metabolic pathways and their regulation. HPLC, mass spectrometry and are key analytical tools combined with biochemical assays.


Keywords

Energy, glucose, Alzheimers, diabetes, astrocytes, glutamate, TCA cycle, amino acids, glycogen, hepatic encephalopathy

Research tools and techniques

mass spectrometry for metabolic mapping
13C H NMR for metabolic mapping
primary cultures of neurons and astrocytes
HPLC


Scientific Personnel

No of Associate Professors/Postdocs: 3
No of PhD students: 2
Other: 3

Key references from within the last 5 years

1. Koivisto, H, Leinonen, H, Puurula, M, Hafez, HS, Barrera, GA, Stridh, MH, Waagepetersen, HS, Tiainen, M, Soininen, P, Zilberter, Y, and Tanila, H. Chronic Pyruvate Supplementation Increases Exploratory Activity and Brain Energy Reserves in Young and Middle-Aged Mice. Front Aging Neurosci, 2016. 8: p. 41.

2. Nissen, JD, Pajecka, K, Stridh, MH, Skytt, DM, and Waagepetersen, HS. Dysfunctional TCA-Cycle Metabolism in Glutamate Dehydrogenase Deficient Astrocytes. Glia, 2015. 63(12): p. 2313-26.

3. Muller, MS, Pedersen, SE, Walls, AB, Waagepetersen, HS, and Bak, LK. Isoform-selective regulation of glycogen phosphorylase by energy deprivation and phosphorylation in astrocytes. Glia, 2015. 63(1): p. 154-62.

4. Karaca, M, Frigerio, F, Migrenne, S, Martin-Levilain, J, Skytt, DM, Pajecka, K, Martin-del-Rio, R, Gruetter, R, Tamarit-Rodriguez, J, Waagepetersen, HS, Magnan, C, and Maechler, P. GDH-Dependent Glutamate Oxidation in the Brain Dictates Peripheral Energy Substrate Distribution. Cell Rep, 2015. 13(2): p. 365-75.

5. Hertz, L, Chen, Y, and Waagepetersen, HS. Effects of ketone bodies in Alzheimer's disease in relation to neural hypometabolism, beta-amyloid toxicity, and astrocyte function. J Neurochem, 2015. 134(1): p. 7-20.

6. Walls, AB, Eyjolfsson, EM, Schousboe, A, Sonnewald, U, and Waagepetersen, HS. A subconvulsive dose of kainate selectively compromises astrocytic metabolism in the mouse brain in vivo. J Cereb Blood Flow Metab, 2014. 34(8): p. 1340-6.

7. Muller, MS, Fox, R, Schousboe, A, Waagepetersen, HS, and Bak, LK. Astrocyte glycogenolysis is triggered by store-operated calcium entry and provides metabolic energy for cellular calcium homeostasis. Glia, 2014. 62(4): p. 526-34.

8. Fries, AW, Dadsetan, S, Keiding, S, Bak, LK, Schousboe, A, Waagepetersen, HS, Simonsen, M, Ott, P, Vilstrup, H, and Sorensen, M. Effect of glutamine synthetase inhibition on brain and interorgan ammonia metabolism in bile duct ligated rats. J Cereb Blood Flow Metab, 2014. 34(3): p. 460-6.

9. Dam, G, Keiding, S, Munk, OL, Ott, P, Vilstrup, H, Bak, LK, Waagepetersen, HS, Schousboe, A, and Sorensen, M. Hepatic encephalopathy is associated with decreased cerebral oxygen metabolism and blood flow, not increased ammonia uptake. Hepatology, 2013. 57(1): p. 258-65.

10. Walls, AB, Eyjolfsson, EM, Smeland, OB, Nilsen, LH, Schousboe, I, Schousboe, A, Sonnewald, U, and Waagepetersen, HS. Knockout of GAD65 has major impact on synaptic GABA synthesized from astrocyte-derived glutamine. J Cereb Blood Flow Metab, 2011. 31(2): p. 494-503.
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2100 Copenhagen Ø
Denmark
Tel. +45 39 12 80 00
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info@thebrainprize.org
www.thebrainprize.org
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